Mass cytometry, or CyTOF, is a powerful technology that has been used for comprehensive multiparameter characterization of immune cells. We recently conducted a 38-parameter characterization of HIV entry and productive infection of tissue CD4+ T cells by CyTOF, and used an analytical approach that takes advantage of the high-dimensional nature of CyTOF datasets to distinguish receptors modulated during infection from those differentially expressed on preferentially infected cells. These studies identified a subset of memory CD4+ T cells susceptible to HIV entry but not productive infection. Ongoing work in the lab seeks to characterize the molecular basis of this post-entry restriction, to use CyTOF and a variety of high-dimensional CyTOF data analysis tools to characterize the types of cells that are productively and latently infected with HIV, and to apply the high-dimensional analysis approaches we have developed on HIV-infected cells characterized by single-cell RNAseq. We are also working closely with UCSF’s SCOPE cohort to identify signatures and biomarkers of infected cells that persist in HIV-infected individuals on suppressive antiretroviral therapy, as well as signatures of immune effector cells that can control viral replication in these infected individuals.
- NIH R01 AI183286 "Targeting the transcriptionally-active reservoir to reduce chronic inflammation in aged people with HIV"
- NIH R21 AI184073 "Targeting CD127-expressing tissue reservoir cells as a strategy for HIV cure"
- NIH R01 AI183286 "Targeting the transcriptionally-active reservoir to reduce chronic inflammation in aged people with HIV"
- NIH R01 DK131526 "Multiomics characterization, induction, and elimination of the HIV gut reservoir"
- NIH R21 AI170166 "Single-cell analysis of glycomic and proteomic features of the HIV reservoir"
- NIH R21 AI172060 "Characterizing ART-free NK cell-mediated control of HIV infection in people living with HIV"
- NIH P01 AI69606 "Reservoir features associated with time-to-rebound during analytical treatment interruption"
- NIH P01 AI169609 "Leaving, Coming, and Staying HIV Obligate Microenvironements (HOME)"
- NIH UM1 AI164567 "CARE - Collaboratory of AIDS Researchers for Eradication"
- NIH UM1 AI164559 "HOPE - HIV Obstruction by Programmed Epigenetics"
- NIH R01 AI147777 "Phenotypic and mechanistic analysis of the in vivo HIV latent reservoir by single-cell technologies"
- NIH P01 AI131374 "Exploiting the Host-HIV interface to identify biomarkers predicting time to viral rebound after treatment interruption"
- NIH R01 AI127219 “Elucidating the mechanism of progesterone-induced permissivity in the upper female reproductive tract”
- George A.F., Luo X., Neidleman J., Hoh, R., Vohra P., Thomas R., Shin M., Lee M.J., Blish C.A., Deeks S.G., Greene W.C., Lee S.A., Roan N.R. Deep phenotypic analysis of blood and lymphoid T and NK cells from HIV controllers and ART-suppressed individuals. 2022. Frontiers in Immunology
- Xie G., Luo X., Ma T., Frouard J., Neidleman J., Hoh R., Deeks S.G., Greene, W.C. Roan N.R. Characterization of HIV-induced remodeling reveals differences in infection susceptibility of memory CD4+ T cell subsets in vivo. 2021. Cell Reports
- Neidleman J., ., Luo X., Frouard J., Xie G., Hsiao F., Ma T., Morcilla V., Lee A., Telwatte S., Thomas R., Tamaki W., Wheeler B., Hoh R., Somsouk M., Vohra P., Milush J., James K., Archin N.M., Hunt P.W., Deeks S.G., Yukl S.A., Palmer S., Greene W.C., Roan N.R. Phenotypic analysis of the unstimulated in vivo HIV CD4 T cell reservoir. 2020. Elife
- Hsiao F., Frouard J., Gramatica A., Guorui X., Telwatte S., Lee G.Q., Roychoudhury P., Schwarzer R., Luo X., Yukl S.A., Lee S., Hoh R., Deeks S.G., Jones R.B., Cavrois M., Greene W.C., Roan N.R. Tissue memory CD4+ T cells expressing the IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection. 2020. PLoS Pathogens
- Cavrois M., Banerjee T., Mukherjee G., Raman N., Hussien R., Rodriguez B.A., Vasquez J., Spitzer M.H., Lazarus N.H., Jones J.J., Ochsenbauer C., McCune J.M., Butcher E.C., Arvin A.M., Sen N., Greene W.C., Roan N.R. Mass cytometric analysis of HIV entry, replication, and remodeling in tissue CD4+ T cells. 2017. Cell Reports
- Roan N.R. and Jakobsen M.R. Friend or foe: Innate sensing of HIV in the female reproductive tract. 2016. Curr HIV/AIDS Rep